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Irene Castellan
PhD student (Nov. 2017 - )

Irene.Castellan2@unibz.it





Title "An innovative management approach for spotted wing drosophila (Drosophila suzukii) using an environmentally friendly attract and kill formulation"

The management of pest insects in the field is an ongoing challenge for agriculture. This challenge becomes even more complex when we have to deal with an invasive pest insect causing increasing damages to local crops. This is the case of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), an invasive fruit fly species arrived in Italy in 2008 from South East Asia, which is threatening fruit farming and viticulture. The peculiarity of this drosophila species lies in its attack modality: females have a pronged, serrated ovipositor that can lay eggs inside the fruit piercing the soft skin surface. The damage indeed, is already visible before the harvest and this makes it difficult to respect pre-harvest intervals with chemical agents. Physical measures are promising (e.g. anti-insect nets), but costly and not always viable. That is why we must rely on a different approach to tackle the problems caused by spotted-wing drosophila (SWD). Chemical ecology approaches allow to resort to instruments that may modify the insect’s behaviour in the field, therefore permitting to improve the control and preventive measures. The aim is to utilize a strategy that will reduce or eliminate the residues of chemical insecticides on the fruits, making the method sustainable. We decided to target yeast volatiles and test their effects on SWD, as they are already known to be interacting with drosophila species in many cases. My PhD research will focus on the chemical ecology of SWD in relation to fruit-yeast volatiles. The main methods utilized are based on headspace analyses to trap the volatile compounds (e.g. direct headspace, CLSA and SPME) and gas chromatography to get deep into the volatile profile characterisation (GC-MS). After this first step, I will investigate how volatiles emitted from yeasts could influence the behaviour of D. suzukii. To accomplish this, the advanced technique of electroantennography will be utilized to test the insects exposing their antennae to the selected volatiles (EAG and GC-EAD). The ultimate aim will be to find a right blend of volatiles to create a formulation potentially applicable in the field to keep the pest presence under control without the use of insecticides. The project will be done in collaboration with Laimburg Research Centre and other national and international institutions.

Last updated 26.11.2017